Director, Todd Kostman
The University of Wisconsin Oshkosh has a long and distinguished history in the teaching of electron microscopy to undergraduate students. The Electron Microscope Facility was begun in 1972 by Dr. Rodney Cyrus, who retired in 1992. Under his direction, the University was among the first undergraduate institutions in the United States to acquire a scanning electron microscope. The Hitachi HHS-2R SEM was a state-of-the-art instrument that was used continuously from 1972 until 1992. An anonymous private donation allowed us to purchase a Hitachi 2460N environmental SEM in February of 1994. That instrument is coupled to a Noran Pioneer energy dispersive spectrometer for complete elemental X-ray microanalysis. Our transmission electron microscope is a Zeiss EM10C 100 kV instrument (funded by an NSF-ILI grant in 1990). Finally, the Spring of 1998 saw the installation of a new Reichert Ultracut UCT ultramicrotome (funded by a USDA equipment grant).
Other equipment includes: LKB 7800 glass knife maker, three diamond knives, LKB 8800 ultramicrotome, Ladd critical point dryer, Polaron E5100 sputter coater, Denton vacuum evaporator, all the chemicals and supplies needed for routine fixation and embedding of biological samples, and a fully equipped photographic dark room with a Durst Laborator 1200 enlarger.
1. Cyrus, R.V., I.Y. Mahmoud and J. Klicka. 1978. Fine structure of the corpus luteum of the snapping turtle, Chelydra serpentina. Copeia 4: 622-627.2. Mahmoud, I.Y., J. Klicka and R. Cyrus. 1980. The rate of conversion of [14C] progesterone by corpia lutea of the snapping turtle, Cheldrya serpentina. General and Comparative Endocrinology 41: 274-277.
3. Mahmoud, I.Y., R.V. Cyrus, T.M. Bennett, M.J. Woller, and D.M. Montag. 1984. Ultrastructural changes in testes of the snapping turtle, Chelydra serpentina, in relation to plasma testosterone, 5-3-hydroxy steroid dehydrogenase, and cholesterol. Journal of Comparative Endrocrinology 57: 454-464.
4. Mahmoud, I.Y., R.V. Cyrus, M.J. Woller, and A. Bieber. 1985. Development of the ovarian follicles in relation to changes in plasma parameters and 5D 3 b HSD in the snapping turtle, Chelydra serpentina. Comparative Biochemistry and Physiology 82A: 131-136.
5. Mahmoud, I.Y., A.E. Colas, M.J. Woller and R.V. Cyrus. 1986. Cytoplasmic progesterone receptors in uterine tissue of the snapping turtle, Cheldrya serpentina. Journal of Endocrinology 109: 385-392.
6. Mahmoud, I.Y., M.E. McAsey, C. Cady, and R.V. Cyrus. 1988. The role of arginine vasotocin and prostagladin F2 on oviposition and luteolysis in the common snapping turtle Chelydra serpentina. General Comparative Endrocrinology 69: 56-64.
7. Mahmoud, I.Y. and R.V. Cyrus. 1992. The testicular cycle of the common snapping turtle, Chelydra serpentina in Wisconsin. Herpetologica 48: 17-22.
8. Mesner, P.W., I.Y. Mahmoud and R.V. Cyrus. 1993. Seasonal testosterone levels in Leydig and Sertoli cells of the snapping turtle (Chelydra serpentina) in natural populations. Journal of Experimental Zoology 266: 266-276.
9. Gibson, C.P. and K.J. Putzer. 1995. Synthesis and characterization of anisometric cobalt nanoclusters. Science 267: 1338-1340.
10. Cushman, K.E., T.W. Tibbitts, T.D. Sharkey and R.R. Wise. 1995. Constant light injury of potato: Temporal and spatial patterns of CO2 assimilation, starch content, chloroplast integrity, and necrosis. The Journal of the American Society of Horticultural Science, 120: 1032-1040.
11. White, S.L. and R.R. Wise. 1998. Anatomy and ultrastructure of Wolffia columbiana and W. borealis (Lemnaceae). International Journal of Plant Sciences, 159: 297-304.
12. Wise, R.R. 1998. High pressure freezing: Benefits and limitations. Microscopy Today 98(9): 14-15.
13. Wise, R.R. and W.C. Cook. 1998. Development of ultrastructural damage to chloroplasts in a plastoquinone-deficient mutant of maize. Environmental and Experimental Botany, 40:221-228.
14. Choinski, J.S. and R.R. Wise. 1999. Leaf growth and development related to gas exchange in Quercus marilandica Muenchh. Journal of Plant Physiology, 154:302-309.
15. McDermott, C.M., C.W. Nho, W. Howard, and B. Holton. The cyanobacterial toxin, microcystin-LR, can induce apoptosis in a variety of mammalian cell types. Toxicon 36: 1981-1996.
16. Kratsch, H.A. and R.R. Wise. 2000. The ultrastructure of chilling stress. Plant, Cell & Environment 23: 337-350.
17. Wise, R.R., G.F. Sassenrath-Cole and R.G. Percy. 2000. A comparison of leaf anatomy in field-grown Gossypium hirsutum and G. Barbadense. Annals of Botany 86: 731-738.
18. Buss, C.C., T. G. Lammers and R.R. Wise. 2000. Seed coat morphology and its systematic implications in Cyanea and other genera of Lobelioideae (Campanulaceae). American Journal of Botany. 88: 1301-1308.
19. Wise, R.R., C.A. Pierstorff, S.L. Nelson, R.M. Bursek, J.L. Plude, M. McNello, and J. Hein. 2001. Morphological deformities in Chironomus (Chironomidae: Diptera) larvae as indicators of pollution in Lake Winnebago, Wisconsin. Journal of Great Lakes Research 27: 503-509.
20. Kostman, T.A., Tarlyn, N.M., Franceschi, V.R. and Loewus, F.A. 2001. Biosynthesis of L-ascorbic acid and conversion of carbons 1 and 2 of L-ascorbic acid to oxalic acid occurs within individual calcium oxalate crystal idioblasts. Plant Physiology 125: 1-7
21. Nekola, J.C. 2001. Distribution and ecology of Vertigo cristata Sterki, 1919 in the Western Great Lakes region. American Malacological Bulletin 16: 47-52
22. Nekola, J.C. and P.A. Massart. 2001. Distribution and ecology of Vertigo nylanderi Sterki, 1090 in the Western Great Lakes region. American Malacological Bulletin 16: 53-60
23. Nekloa, J.C. and B.F. Coles. 2001. Systematics and ecology of Gastrocopta (Gastrocopta) rogersensis (Gastropoda: Pupillidae), a new species of land snail from the Midwest of the United States of America. The Nautilus 115: 105-114
24. Wise, R.R. 2002. Inexpensive holder for hand-trimming resin-embedded tissues. Microscopy Research and Technique 56: 306-307
25. Rozak, P.R., R.M. Seiser, W.F. Wacholtz, and R.R. Wise. 2002. Rapid, reversible alterations in spinach thylakoid appression upon changes in light intensity. Plant, Cell & Environment 25: 421-429
26. Volk, G.M., Lynch-Holm, V.J., Kostman, T.A., Goss, L.J., and Franceschi V.R. 2002. The role of druse and raphide calcium oxalate crystals in tissue calcium regulation in Pistia stratiotes leaves. Plant Biology 4: 34-45.
27. Vaughan D.K., Nemke J., Fliesler S.J., Darrow R. and Organisciak D.T. 2002. Evidence for a circadian rhythm of light damage susceptibility. Photochemistry and Photobiology 75:547-553
28. Kostman, T.A.; Franceschi, V.R.; Nakata, P.A. 2003. Endoplasmic reticulum sub-compartments are involved in calcium sequestration within raphide crystal idioblasts of Pistia stratiotes L. Plant Science 165: 205-212
29. Kostman T.A. and J.R. Koscher. 2003. L-galctono- g -lactone dehydrogenase is present in calcium oxalate crystal idioblasts of two plant species. Plant Physiology and Biochemistry 41: 201-206
30. Vaughan D.K., Coulibaly S., Darrow R.M. and Organisciak D.T. 2003. A morphometric study of light damage in transgenic rat models of retinitis pigmentosa. Investigations in Opthalmolic and Vision Science 44:856-865
31. Hoey, K.A., R.R. Wise, and G.H. Adler. 2004. Ultrastructure of echimyid and murid rodent spines. Journal of Zoology, London 263: 307-315
32. Batterman, L.R.W. and T.G. Lammers. 2004. Branched foliar trichomes of Lobelioideae (Campanulaceae) and the infragenic classification of Centropogon. Systematic Botany 29: 448-458
33. Fliesle S.J., Peachey N.S, Richards M.J., Nagel B.A. and Vaughan D.K. 2004. Retinal degeneration in a rodent model of Smith-Lemli-Opitz Syndrome: Electrophysiological, biochemical, and morphological features. Archives of Ophthalmology 122:1190-1200
34. Wise, R.R. 2005. The diversity of plastid form and function. In: Wise, R.R. and J.K. Hoober. The Structure and Function of Plastids, Springer, Dordrecht. In press.
1. Woller, Michael. 1984. Localization and characterization of progesterone receptors in the uterus and oviduct of the common snapping turtle, Chelydra serpentina. Masters Thesis, U.W. Oshkosh
2. Hofman, Gordon. 1996. Anatomical and ultrastructural investigations of Gigaspora rosea. Masters Thesis, U.W. Oshkosh.
3. Seiser, Robert 1996. The state transition: Theory, mechanism, and application in higher plants. Honors Thesis, Lawrence University, Appleton, WI.
4. Clancy, Yevette. 1996. Isolation and characterization of a methanogenic bacterium from the rabbit cecum. Masters Thesis, U.W. Oshkosh
5. Heil, Paulette 1997. Seasonal changes in the glandular tissues of the reproductive tract of the common snapping turtle, Cheldrya serpentina. Masters Thesis, U.W. Oshkosh.
6. Hoey, Kelley. 1998. External spine morphology of the genus Proechimys (Rodentia: Echimydae) as revealed by scanning electron microscopy. Masters Thesis, U.W. Oshkosh.
7. Kratsch, Heidi. 2000. Evidence for actin-based chloroplast movement in leaves of Pisum sativum (garden pea). Masters Thesis, U.W. Oshkosh.
8. Rozak, Phil 2000. Alterations in thylakoid membrane ultrastructure of spinach (Spinacia oleracea L.) accompany State changes. Masters Thesis, U.W. Oshkosh.
9. Fruend, Jennifer. 2000. Cold temperature and light-induced chloroplast movement in chilling-sensitive and chilling-resistant plants . Masters Thesis, U.W. Oshkosh.
10. Nemke, Jennifer L. 2001. Morphological evidence for a circadian rhythm of susceptibility to retinal light damage. Masters Thesis, U.W. Oshkosh.
11. Batterman, McLynda. 2002. Trichome morphology and its systematic implications in Centropogn and other lobelioideae ( Campanulaceae ) genera. Masters Thesis, U.W. Oshkosh.
12. Jenewien, Erin. 2005. Rodent retina models of an inborn error of sterol metabolism. Masters Thesis, U.W. Oshkosh.
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Last updated 14 July 2005